University of Exeter Medical School

James Crichton

James Crichton

Experimental Officer (Imaging and Image Analysis)

 J.C.Crichton@exeter.ac.uk

 RILD Building Level 4

 

University of Exeter Medical School, RILD Building, RD&E Hospital Wonford, Barrack Road, Exeter, EX2 5DW, UK

Overview

I am an imaging scientist based in the RILD building, where I support the use of imaging technology in biomedical research. I manage our local microscopy suite and collaborate with users to develop experimental solutions to facilitate their research. I am passionate about bioimage analysis and enjoy extracting quantitative data from images to drive forward scientific discovery.

My PhD and postdoc at The University of Edinburgh involved studying mechanisms by which the genome is shuffled, protected, and sometimes attacked, during the production of sperm and eggs, to ensure the sharing of high-quality genetically varied DNA with offspring. Fluorescence microscopy was a central tool in my research, using super-resolution techniques to capture nuclear processes in ever-more detail, followed by the development of computational tools to enable quantitative exploration of these complex processes.

Qualifications

  • BSc Biological Sciences, University of Warwick
  • MSc Human Molecular Genetics, Imperial College London
  • PhD Genome Stability, University of Edinburgh

Back to top

Publications

Journal articles

Crichton JH, Dunce JM, Dunne OM, Salmon LJ, Devenney PS, Lawson J, Adams IR, Davies OR (2023). Structural maturation of SYCP1-mediated meiotic chromosome synapsis by SYCE3. Nature Structural & Molecular Biology, 30(2), 188-199.
Reichmann J, Dobie K, Lister LM, Crichton JH, Best D, MacLennan M, Read D, Raymond ES, Hung C-C, Boyle S, et al (2020). <i>Tex19.1</i> inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. Journal of Cell Biology, 219(5). Abstract.
Ehrmann I, Crichton JH, Gazzara MR, James K, Liu Y, Grellscheid SN, Curk T, de Rooij D, Steyn JS, Cockell S, et al (2019). An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. eLife, 8 Abstract.
Rosario R, Crichton JH, Stewart HL, Childs AJ, Adams IR, Anderson RA (2019). Dazl determines primordial follicle formation through the translational regulation of Tex14. FASEB Journal, 33(12), 14221-14233. Abstract.
Crichton JH, Read D, Adams IR (2018). Defects in meiotic recombination delay progression through pachytene in Tex19.1−/− mouse spermatocytes. Chromosoma, 127(4), 437-459.
Crichton JH, Playfoot CJ, MacLennan M, Read D, Cooke HJ, Adams IR (2017). Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes. PLOS Genetics, 13(7), e1006904-e1006904.
MacLennan M, Crichton JH, Playfoot CJ, Adams IR (2015). Oocyte development, meiosis and aneuploidy. Seminars in Cell & Developmental Biology, 45, 68-76.
Crichton JH, Playfoot CJ, Adams IR (2014). The Role of Chromatin Modifications in Progression through Mouse Meiotic Prophase. Journal of Genetics and Genomics, 41(3), 97-106.
Crichton JH, Dunican DS, MacLennan M, Meehan RR, Adams IR (2013). Defending the genome from the enemy within: mechanisms of retrotransposon suppression in the mouse germline. Cellular and Molecular Life Sciences, 71(9), 1581-1605.
Reichmann J, Crichton JH, Madej MJ, Taggart M, Gautier P, Garcia-Perez JL, Meehan RR, Adams IR (2012). Microarray Analysis of LTR Retrotransposon Silencing Identifies Hdac1 as a Regulator of Retrotransposon Expression in Mouse Embryonic Stem Cells. PLoS Computational Biology, 8(4), e1002486-e1002486.

Back to top

Edit Profile