Publications by category
Journal articles
Davies M, Bramwell LR, Jeffery N, Bunce B, Lee BP, Knight B, Auckland C, Masoli JA, Harries LW (2022). Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
Int J Infect Dis,
116, 418-425.
Abstract:
Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
OBJECTIVES: This study aimed to evaluate the associations between COVID-19 severity and active viral load, and to characterize the dynamics of active SARS-CoV-2 clearance in a series of archival samples taken from patients in the first wave of COVID-19 infection in the South West of the UK. METHODS: Subgenomic RNA (sgRNA) and E-gene genomic sequences were measured in a retrospective collection of PCR-confirmed SARS-CoV-2-positive samples from 176 individuals, and related to disease severity. Viral clearance dynamics were then assessed in relation to symptom onset and last positive test. RESULTS: Whilst E-gene sgRNAs declined before E-gene genomic sequences, some individuals retained sgRNA positivity for up to 68 days. 13% of sgRNA-positive cases still exhibited clinically relevant levels of virus after 10 days, with no clinical features previously associated with prolonged viral clearance times. CONCLUSIONS: Our results suggest that potentially active virus can sometimes persist beyond a 10-day period, and could pose a potential risk of onward transmission. Where this would pose a serious public health threat, additional mitigation strategies may be necessary to reduce the risk of secondary cases in vulnerable settings.
Abstract.
Author URL.
Bramwell LR, Harries LW (2021). Targeting Alternative Splicing for Reversal of Cellular Senescence in the Context of Aesthetic Aging.
Plast Reconstr Surg,
147(1S-2), 25S-32S.
Abstract:
Targeting Alternative Splicing for Reversal of Cellular Senescence in the Context of Aesthetic Aging.
Cellular senescence is a state of stable cell cycle arrest that has increasingly been linked with cellular, tissue, and organismal aging; targeted removal of senescent cells brings healthspan and lifespan benefits in animal models. Newly emerging approaches to specifically ablate or rejuvenate senescent cells are now the subject of intense study to explore their utility to provide novel treatments for the aesthetic signs and diseases of aging in humans. Here, we discuss different strategies that are being trialed in vitro, and more recently in vivo, for the targeted removal or reversal of senescent cells. Finally, we describe the evidence for a newly emerging molecular mechanism that may underpin senescence; dysregulation of alternative splicing. We will explore the potential of restoring splicing regulation as a novel "senotherapeutic" approach and discuss strategies by which this could be integrated into the established portfolio of skin aging therapeutics.
Abstract.
Author URL.
McCulloch LJ, Bramwell LR, Knight B, Kos K (2020). Circulating and tissue specific transcription of angiopoietin-like protein 4 in human Type 2 diabetes. Metabolism, 106, 154192-154192.
Ayyappa KA, Shatwan I, Bodhini D, Bramwell LR, Ramya K, Sudha V, Anjana RM, Lovegrove JA, Mohan V, Radha V, et al (2017). High fat diet modifies the association of lipoprotein lipase gene polymorphism with high density lipoprotein cholesterol in an Asian Indian population. Nutrition & Metabolism, 14(1).
Publications by year
2023
Bramwell L, Gould S, Davies M, Trusler E, McMullan C, Harries L (2023). An evaluation of the replacement of animal-derived biomaterials in human primary tissue culture.
2022
Davies M, Bramwell LR, Jeffery N, Bunce B, Lee BP, Knight B, Auckland C, Masoli JA, Harries LW (2022). Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
Int J Infect Dis,
116, 418-425.
Abstract:
Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
OBJECTIVES: This study aimed to evaluate the associations between COVID-19 severity and active viral load, and to characterize the dynamics of active SARS-CoV-2 clearance in a series of archival samples taken from patients in the first wave of COVID-19 infection in the South West of the UK. METHODS: Subgenomic RNA (sgRNA) and E-gene genomic sequences were measured in a retrospective collection of PCR-confirmed SARS-CoV-2-positive samples from 176 individuals, and related to disease severity. Viral clearance dynamics were then assessed in relation to symptom onset and last positive test. RESULTS: Whilst E-gene sgRNAs declined before E-gene genomic sequences, some individuals retained sgRNA positivity for up to 68 days. 13% of sgRNA-positive cases still exhibited clinically relevant levels of virus after 10 days, with no clinical features previously associated with prolonged viral clearance times. CONCLUSIONS: Our results suggest that potentially active virus can sometimes persist beyond a 10-day period, and could pose a potential risk of onward transmission. Where this would pose a serious public health threat, additional mitigation strategies may be necessary to reduce the risk of secondary cases in vulnerable settings.
Abstract.
Author URL.
2021
Bramwell LR, Harries LW (2021). Targeting Alternative Splicing for Reversal of Cellular Senescence in the Context of Aesthetic Aging.
Plast Reconstr Surg,
147(1S-2), 25S-32S.
Abstract:
Targeting Alternative Splicing for Reversal of Cellular Senescence in the Context of Aesthetic Aging.
Cellular senescence is a state of stable cell cycle arrest that has increasingly been linked with cellular, tissue, and organismal aging; targeted removal of senescent cells brings healthspan and lifespan benefits in animal models. Newly emerging approaches to specifically ablate or rejuvenate senescent cells are now the subject of intense study to explore their utility to provide novel treatments for the aesthetic signs and diseases of aging in humans. Here, we discuss different strategies that are being trialed in vitro, and more recently in vivo, for the targeted removal or reversal of senescent cells. Finally, we describe the evidence for a newly emerging molecular mechanism that may underpin senescence; dysregulation of alternative splicing. We will explore the potential of restoring splicing regulation as a novel "senotherapeutic" approach and discuss strategies by which this could be integrated into the established portfolio of skin aging therapeutics.
Abstract.
Author URL.
2020
McCulloch LJ, Bramwell LR, Knight B, Kos K (2020). Circulating and tissue specific transcription of angiopoietin-like protein 4 in human Type 2 diabetes. Metabolism, 106, 154192-154192.
2017
Ayyappa KA, Shatwan I, Bodhini D, Bramwell LR, Ramya K, Sudha V, Anjana RM, Lovegrove JA, Mohan V, Radha V, et al (2017). High fat diet modifies the association of lipoprotein lipase gene polymorphism with high density lipoprotein cholesterol in an Asian Indian population. Nutrition & Metabolism, 14(1).
