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Journal articles
Dogra Y, Ferguson DCJ, Dodd NJF, Smerdon GR, Curnow A, Winyard PG (2016). The hydroxypyridinone iron chelator CP94 increases methyl-aminolevulinate-based photodynamic cell killing by increasing the generation of reactive oxygen species.
Redox Biol,
9, 90-99.
Abstract:
The hydroxypyridinone iron chelator CP94 increases methyl-aminolevulinate-based photodynamic cell killing by increasing the generation of reactive oxygen species.
Methyl-aminolevulinate-based photodynamic therapy (MAL-PDT) is utilised clinically for the treatment of non-melanoma skin cancers and pre-cancers and the hydroxypyridinone iron chelator, CP94, has successfully been demonstrated to increase MAL-PDT efficacy in an initial clinical pilot study. However, the biochemical and photochemical processes leading to CP94-enhanced photodynamic cell death, beyond the well-documented increases in accumulation of the photosensitiser protoporphyrin IX (PpIX), have not yet been fully elucidated. This investigation demonstrated that MAL-based photodynamic cell killing of cultured human squamous carcinoma cells (A431) occurred in a predominantly necrotic manner following the generation of singlet oxygen and ROS. Augmenting MAL-based photodynamic cell killing with CP94 co-treatment resulted in increased PpIX accumulation, MitoSOX-detectable ROS generation (probably of mitochondrial origin) and necrotic cell death, but did not affect singlet oxygen generation. We also report (to our knowledge, for the first time) the detection of intracellular PpIX-generated singlet oxygen in whole cells via electron paramagnetic resonance spectroscopy in conjunction with a spin trap.
Abstract.
Author URL.
Full text.
Ferguson D, Smerdon GR, Eggleton P, Curnow A, Winyard PG (2012). Altering oxygen concentrations to enhance the efficacy of PpIX-based photodynamic cell killing.
FREE RADICAL BIOLOGY AND MEDICINE,
53, S127-S127.
Author URL.
Kendall AC, Whatmore JL, Harries LW, Winyard PG, Ferguson D, Eggleton P (2012). Different oxygen treatment pressures alter oxygen responses and redox signalling gene expression in human endothelial cells.
FREE RADICAL BIOLOGY AND MEDICINE,
53, S166-S166.
Author URL.
Conferences
Ferguson D, Perry A, Wood ME, Winyard PG, Whiteman M (2014). Potentiation of Methyl Aminolevulinate (MAL)-Induced Photodynamic Therapy (PDT) Killing of Skin Cancer Cells by Mitochondria-Targeted Hydrogen Sulfide (H2S) Donors.
Author URL.
Publications by year
2016
Dogra Y, Ferguson DCJ, Dodd NJF, Smerdon GR, Curnow A, Winyard PG (2016). The hydroxypyridinone iron chelator CP94 increases methyl-aminolevulinate-based photodynamic cell killing by increasing the generation of reactive oxygen species.
Redox Biol,
9, 90-99.
Abstract:
The hydroxypyridinone iron chelator CP94 increases methyl-aminolevulinate-based photodynamic cell killing by increasing the generation of reactive oxygen species.
Methyl-aminolevulinate-based photodynamic therapy (MAL-PDT) is utilised clinically for the treatment of non-melanoma skin cancers and pre-cancers and the hydroxypyridinone iron chelator, CP94, has successfully been demonstrated to increase MAL-PDT efficacy in an initial clinical pilot study. However, the biochemical and photochemical processes leading to CP94-enhanced photodynamic cell death, beyond the well-documented increases in accumulation of the photosensitiser protoporphyrin IX (PpIX), have not yet been fully elucidated. This investigation demonstrated that MAL-based photodynamic cell killing of cultured human squamous carcinoma cells (A431) occurred in a predominantly necrotic manner following the generation of singlet oxygen and ROS. Augmenting MAL-based photodynamic cell killing with CP94 co-treatment resulted in increased PpIX accumulation, MitoSOX-detectable ROS generation (probably of mitochondrial origin) and necrotic cell death, but did not affect singlet oxygen generation. We also report (to our knowledge, for the first time) the detection of intracellular PpIX-generated singlet oxygen in whole cells via electron paramagnetic resonance spectroscopy in conjunction with a spin trap.
Abstract.
Author URL.
Full text.
2014
Ferguson D, Perry A, Wood ME, Winyard PG, Whiteman M (2014). Potentiation of Methyl Aminolevulinate (MAL)-Induced Photodynamic Therapy (PDT) Killing of Skin Cancer Cells by Mitochondria-Targeted Hydrogen Sulfide (H2S) Donors.
Author URL.
2012
Ferguson D, Smerdon GR, Eggleton P, Curnow A, Winyard PG (2012). Altering oxygen concentrations to enhance the efficacy of PpIX-based photodynamic cell killing.
FREE RADICAL BIOLOGY AND MEDICINE,
53, S127-S127.
Author URL.
Kendall AC, Whatmore JL, Harries LW, Winyard PG, Ferguson D, Eggleton P (2012). Different oxygen treatment pressures alter oxygen responses and redox signalling gene expression in human endothelial cells.
FREE RADICAL BIOLOGY AND MEDICINE,
53, S166-S166.
Author URL.
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