COLLEGE OF MEDICINE AND HEALTH
Medicine, Nursing and Allied Health Professions

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Dr Catalin Chimerel

Dr Catalin Chimerel

Research Fellow

 7481

 +44 (0)1392 727481

 Living Systems Institute T02.14

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

We are looking for a motivated PhD student and a Research Technician! Positions are available in my group starting from September 2019. Please contact me for further information.

 

Currently I am a Independent Research Fellow in the Living Systems Institute at the University of Exeter. I have been formally trained as a Physicist but my research has developed at the interface between Physics and Biology. 

Qualifications

PhD, Physics, Cavendish Laboratory, University of Cambridge, Thesis Title: Passive transport through biological membranes (supervisor, Professor Ulrich Keyser), 2013

Master’s Degree in the Nanomolecular Science, Jacobs University Bremen, Germany, 2008.

Bachelor of Science in Physics, Jacobs University Bremen, Germany, 2007.

Career

My research career embraced a transdisciplinary path and progressed from understanding fundamental cellular processes to organism function.

I have started  my research career by investigating fundamentals of membrane biophysics during my PhD when I have discovered that the bacterial signal indole is a natural ionophore regulating a key biological process in bacteria (C. Chimerel et al., 2012, Biochemica and Biophysica Acta‑Membranes). Subsequently I pursued the effect of indole on mitochondria and I have shown that indole is capable to uncouple oxidative phosphorylation from ATP production revealing a potential link between the prokaryotes and eukaryotes (C. Chimerel et al., 2013, ChemPhysChem). I then recognised that indole is produced by bacteria in the human gut in miliMolar concentrations and I speculated it could affect the functioning of the cells in the gut epithelium via it ionophoric properties. Starting from this hypothesis I proved that indole modulates the secretion of gut hormones from the enteroendocrine cells in the gut epithelium in vitro and I have revealed the molecular details behind this modulation (C. Chimerel et al., 2014, Cell Reports).

I have been involved with other research projects in the past with a special focus on the passive diffusion through biological membranes. I started working on this topic while studying for my BSc Degree when I demonstrated that a membrane porin, Outer Membrane Protein F (OmpF), embedded in a lipid membrane is conformationally stable on a broad temperature range: 0⁰ to 90⁰C (C. Chimerel et al., 2008, European Biophys. J.). Analysing the temperature dependence of the ionic current flowing through OmpF I developed a better understanding of the physics of ionic transport in confinement (C. Chimerel et al., 2008, European Biophys. J.) and investigated the kinetics of antibiotics passing through membrane porins (K. R. Mahendran, C. Chimerel et al., 2009, European Biophys. J.). During my PhD I have complemented this expertise by developing protocols to reconstitute membrane porins in nanolipidbilayers supported on glass capillaries and I investigated the passive diffusion of molecules with intrinsic fluorescence (e.g. indole, norfloxacin). These experiments clarified an overlooked transport pathway for the bacterial signalling molecule indole passing through lipid membranes (S. Pinero-Fernandez, C. Chimerel al., 2011, J. of Bacteriology). Furthermore I have developed a novel, label-free optofluidic assay, which quantifies the permeability coefficient of auto fluorescent antibiotic molecules permeating through cellular membranes (J. Cama et al. 2014 Lab Chip).

 

 

Research group links

Research

Research interests

Our research group focuses on problems of molecular transport across cellular membranes. We are particularly interested and motivated to provide answers for biomedical questions such as the problem of drug permeation through cellular membranes. Moreover we are keen to develop new technological tools for a better understanding of molecular transport.  

Research projects

1. Drug Permeation in Cells

Drugs entering cells are routinely quantified by lysing the cell and measuring drug levels in the cell lysate, for example by fluorescence spectroscopy or mass spectrometry. Although this method provides important insights into the problem of drug permeation it is restricted to cell‑population analysis and lacks the time resolution. In this project we are developing a novel single cell spectrometer designed to quantify the spectral fingerprint of drugs absorbed in cells. As a biological model we are currently using bacteria and antibiotics to validate these measurements.

References:

1. J. Cama, C. Chimerel, S. Pagliara, A. Javer, U. F. Keyser. A Label-Free Microfluidic Assay to quantitatively study antibiotic diffusion through lipid membranes. 2014, LAB ON A CHIP

2. K. R. Mahendran, C. Chimerel, T. Mach, M. Winterhalter. Antibiotic translocation through membrane channels: temperature-dependent ion current fluctuation for catching the fast events. 2009, EUROPEAN BIOPHYSICS JOURNAL.

Funded by: Academy of Medical Sciences Springboard Grant and University of Exeter

2. Multidimensional Cell Sorting

Historically cells have been catalogued in respect to their architecture by using fluorescent dyes to specifically label cellular and subcellular structures. Cell shape, organelle distribution and spatial localisation within the tissue have all been used to distinguish cell types. More recently, antibodies linked with fluorescent markers that can bind to individual molecules have been developed and used to differentiate cell types. However, such approaches are inaccurate, severely limited in their application to live cells and are difficult to use for classifying and separating cells based on functional phenotypic responses. In this project we are developing an optofluidc platform which uses cell function to differentiate cell types. By analysing in real time the cell responses to specific stimuli, the device isolates from a mixture cells that specifically respond to one or more stimuli.

References:

1. C. Chimerel, C. Riccio, K. Murison, F. M. Gribble and F. Reimann. Optogenetic Analysis of Depolarization-Dependent Glucagonlike Peptide-1 Release. 2017, ENDOCRYNOLOGY.

2. C. Chimerel, E. Emery, D. K. Summers, U. F. Keyser, F. M. Gribble and F. Reimann. Bacteria-specific metabolite of tryptophan, indole, modulates incretin secretion from intestinal enteroendocrine L-cells. 2014, CELL REPORTS

Funded by: BBSRC BB/R022127/1 Research Grant and University of Exeter

 

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Haynes CJE, Zhu J, Chimerel C, Hernández-Ainsa S, Riddell IA, Ronson TK, Keyser UF, Nitschke JR (In Press). Blockable Zn10 L15 Ion Channels through Subcomponent Self-Assembly. Angew Chem Int Ed Engl, 56(48), 15388-15392. Abstract.  Author URL.
Chimerel C, Riccio C, Murison K, Gribble FM, Reimann F (In Press). Optogenetic Analysis of Depolarization-Dependent Glucagonlike Peptide-1 Release. Endocrinology, 158(10), 3426-3434. Abstract.  Author URL.
Cama J, Chimerel C, Pagliara S, Javer A, Keyser UF (2014). A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes. Lab Chip, 14(13), 2303-2308. Abstract.  Author URL.  Full text.
Chimerel C, Emery E, Summers DK, Keyser U, Gribble FM, Reimann F (2014). Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells. Cell Rep, 9(4), 1202-1208. Abstract.  Author URL.
Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF (2013). Detecting DNA Folding with Nanocapillaries (vol 10, pg 2493, 2010). NANO LETTERS, 13(7), 3444-3444. Author URL.
Chimerel C, Murray AJ, Oldewurtel ER, Summers DK, Keyser UF (2013). The effect of bacterial signal indole on the electrical properties of lipid membranes. Chemphyschem, 14(2), 417-423. Abstract.  Author URL.
Chimerel C, Field CM, Piñero-Fernandez S, Keyser UF, Summers DK (2012). Indole prevents Escherichia coli cell division by modulating membrane potential. Biochim Biophys Acta, 1818(7), 1590-1594. Abstract.  Author URL.
Pagliara S, Chimerel C, Aarts DGAL, Langford R, Keyser UF (2011). Colloid flow control in microchannels and detection by laser scattering. Progress in Colloid and Polymer Science, 139, 45-49. Abstract.
Piñero-Fernandez S, Chimerel C, Keyser UF, Summers DK (2011). Indole transport across Escherichia coli membranes. J Bacteriol, 193(8), 1793-1798. Abstract.  Author URL.
Pagliara S, Chimerel C, Langford R, Aarts DGAL, Keyser UF (2011). Parallel sub-micrometre channels with different dimensions for laser scattering detection. Lab Chip, 11(19), 3365-3368. Abstract.  Author URL.
Gornall JL, Mahendran KR, Pambos OJ, Steinbock LJ, Otto O, Chimerel C, Winterhalter M, Keyser UF (2011). Simple reconstitution of protein pores in nano lipid bilayers. Nano Lett, 11(8), 3334-3340. Abstract.  Author URL.
Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF (2010). Detecting DNA folding with nanocapillaries. Nano Lett, 10(7), 2493-2497. Abstract.  Author URL.
Steinbock LJ, Otto O, Skarstam DR, Jahn S, Chimerel C, Gornall JL, Keyser UF (2010). Probing DNA with micro- and nanocapillaries and optical tweezers. J Phys Condens Matter, 22(45). Abstract.  Author URL.
Mahendran KR, Chimerel C, Mach T, Winterhalter M (2009). Antibiotic translocation through membrane channels: temperature-dependent ion current fluctuation for catching the fast events. Eur Biophys J, 38(8), 1141-1145. Abstract.  Author URL.
Pezeshki S, Chimerel C, Bessonov AN, Winterhalter M, Kleinekathöfer U (2009). Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpF. Biophys J, 97(7), 1898-1906. Abstract.  Author URL.
Mach T, Chimerel C, Fritz J, Fertig N, Winterhalter M, Fütterer C (2008). Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion. Anal Bioanal Chem, 390(3), 841-846. Abstract.  Author URL.
Chimerel C, Movileanu L, Pezeshki S, Winterhalter M, Kleinekathöfer U (2008). Transport at the nanoscale: temperature dependence of ion conductance. Eur Biophys J, 38(1), 121-125. Abstract.  Author URL.

Conferences

Chimerel C, Ainsa SMH, Cama J, Summers DK, Keyser UF (2013). Bacterial Signal Indole Modifies the Physicochemical Properties of Lipid Membranes.  Author URL.
Chimerel C, Field CM, Pinero-Fernandez S, Hernandez-Ainsa SM, Summers DK, Keyser UF (2012). Escherichia Coli Regulates Cell Division by Modulating Membrane Potential.  Author URL.
Chimerel C, Keyser UF, Pinero-Fernandez S, Summers DK (2011). Indole Transport across Escherichia Coli Membranes.  Author URL.
Kleinekathoefer U, Pezeshki S, Chimerel C, Movileanu L, Winterhalter M (2009). Ion Transport through OmpF in Molecular Dynamics Simulations and Experiments.  Author URL.
Chimerel C, Movileanu L, Kleinekathofer U, Winterhatter M (2007). Temperature dependent conductance of OmpF: single channel recording and molecular dynamics.  Author URL.

Publications by year


In Press

Haynes CJE, Zhu J, Chimerel C, Hernández-Ainsa S, Riddell IA, Ronson TK, Keyser UF, Nitschke JR (In Press). Blockable Zn10 L15 Ion Channels through Subcomponent Self-Assembly. Angew Chem Int Ed Engl, 56(48), 15388-15392. Abstract.  Author URL.
Chimerel C, Riccio C, Murison K, Gribble FM, Reimann F (In Press). Optogenetic Analysis of Depolarization-Dependent Glucagonlike Peptide-1 Release. Endocrinology, 158(10), 3426-3434. Abstract.  Author URL.

2014

Cama J, Chimerel C, Pagliara S, Javer A, Keyser UF (2014). A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes. Lab Chip, 14(13), 2303-2308. Abstract.  Author URL.  Full text.
Chimerel C, Emery E, Summers DK, Keyser U, Gribble FM, Reimann F (2014). Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells. Cell Rep, 9(4), 1202-1208. Abstract.  Author URL.

2013

Chimerel C, Ainsa SMH, Cama J, Summers DK, Keyser UF (2013). Bacterial Signal Indole Modifies the Physicochemical Properties of Lipid Membranes.  Author URL.
Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF (2013). Detecting DNA Folding with Nanocapillaries (vol 10, pg 2493, 2010). NANO LETTERS, 13(7), 3444-3444. Author URL.
Chimerel C, Murray AJ, Oldewurtel ER, Summers DK, Keyser UF (2013). The effect of bacterial signal indole on the electrical properties of lipid membranes. Chemphyschem, 14(2), 417-423. Abstract.  Author URL.

2012

Chimerel C, Field CM, Pinero-Fernandez S, Hernandez-Ainsa SM, Summers DK, Keyser UF (2012). Escherichia Coli Regulates Cell Division by Modulating Membrane Potential.  Author URL.
Chimerel C, Field CM, Piñero-Fernandez S, Keyser UF, Summers DK (2012). Indole prevents Escherichia coli cell division by modulating membrane potential. Biochim Biophys Acta, 1818(7), 1590-1594. Abstract.  Author URL.

2011

Pagliara S, Chimerel C, Aarts DGAL, Langford R, Keyser UF (2011). Colloid flow control in microchannels and detection by laser scattering. Progress in Colloid and Polymer Science, 139, 45-49. Abstract.
Chimerel C, Keyser UF, Pinero-Fernandez S, Summers DK (2011). Indole Transport across Escherichia Coli Membranes.  Author URL.
Piñero-Fernandez S, Chimerel C, Keyser UF, Summers DK (2011). Indole transport across Escherichia coli membranes. J Bacteriol, 193(8), 1793-1798. Abstract.  Author URL.
Pagliara S, Chimerel C, Langford R, Aarts DGAL, Keyser UF (2011). Parallel sub-micrometre channels with different dimensions for laser scattering detection. Lab Chip, 11(19), 3365-3368. Abstract.  Author URL.
Gornall JL, Mahendran KR, Pambos OJ, Steinbock LJ, Otto O, Chimerel C, Winterhalter M, Keyser UF (2011). Simple reconstitution of protein pores in nano lipid bilayers. Nano Lett, 11(8), 3334-3340. Abstract.  Author URL.

2010

Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF (2010). Detecting DNA folding with nanocapillaries. Nano Lett, 10(7), 2493-2497. Abstract.  Author URL.
Steinbock LJ, Otto O, Skarstam DR, Jahn S, Chimerel C, Gornall JL, Keyser UF (2010). Probing DNA with micro- and nanocapillaries and optical tweezers. J Phys Condens Matter, 22(45). Abstract.  Author URL.

2009

Mahendran KR, Chimerel C, Mach T, Winterhalter M (2009). Antibiotic translocation through membrane channels: temperature-dependent ion current fluctuation for catching the fast events. Eur Biophys J, 38(8), 1141-1145. Abstract.  Author URL.
Kleinekathoefer U, Pezeshki S, Chimerel C, Movileanu L, Winterhalter M (2009). Ion Transport through OmpF in Molecular Dynamics Simulations and Experiments.  Author URL.
Pezeshki S, Chimerel C, Bessonov AN, Winterhalter M, Kleinekathöfer U (2009). Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpF. Biophys J, 97(7), 1898-1906. Abstract.  Author URL.

2008

Mach T, Chimerel C, Fritz J, Fertig N, Winterhalter M, Fütterer C (2008). Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion. Anal Bioanal Chem, 390(3), 841-846. Abstract.  Author URL.
Chimerel C, Movileanu L, Pezeshki S, Winterhalter M, Kleinekathöfer U (2008). Transport at the nanoscale: temperature dependence of ion conductance. Eur Biophys J, 38(1), 121-125. Abstract.  Author URL.

2007

Chimerel C, Movileanu L, Kleinekathofer U, Winterhatter M (2007). Temperature dependent conductance of OmpF: single channel recording and molecular dynamics.  Author URL.

Catalin_Chimerel Details from cache as at 2019-08-21 18:36:51

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Teaching

Supervision / Group

Postdoctoral researchers

  • Petar Nikiforov

Research Technicians

  • Imogen Fermor

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